# Changeset 3

Ignore:
Timestamp:
Jun 18, 2009 6:21:20 AM (10 years ago)
Message:

Created \mbox to protect hyphen between 400 and 500 uL (first paragraph).

File:
1 edited

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 r1 \chapter{Cell and Rotor loading}\label{cellandrotorloading} Figure \ref{fig-cltsc} panel A shows two different, accepted methods of loading cells. For pipetting, the user can piggy-back (stacking) at round, gel-loading tip onto a 1000 $\mu\text{L}$ tip to load 400500 $\mu\text{L}$ of reference buffer or sample. Air must be allowed to escape from the chambers as fluid is pushed into the centerpiece; otherwise, the fluid will be pushed out. Concentrated samples and those containing detergents are more difficult to load. A continual, slow injection will help to prevent problems. Once the reference buffer and/or samples are loaded, a small gasket covers the hole and a small brass screw is placed in the hole and tightened (Figure \ref{fig-cltsc} panel B). Over-tightening of the brass screw can cause warping of the cell housing and make it difficult to push into the rotor hole.\begin{figure}[htbp]\begin{center} Figure \ref{fig-cltsc} panel A shows two different, accepted methods of loading cells. For pipetting, the user can piggy-back (stacking) a round gel-loading tip onto a 1000 $\mu\text{L}$ tip to load \mbox{400-500} $\mu\text{L}$ of reference buffer or sample solution. Air must be allowed to escape from the chambers as fluid is pushed into the centerpiece; otherwise, the fluid will be pushed out. Concentrated samples and those containing detergents are more difficult to load. A continual, slow injection will help to prevent problems. Once the reference buffer and/or samples are loaded, a small gasket covers the hole and a small brass screw is placed in the hole and tightened (Figure \ref{fig-cltsc} panel B). Over-tightening of the brass screw can cause warping of the cell housing and make it difficult to push into the rotor hole.\begin{figure}[htbp]\begin{center} \includegraphics[width=0.9\linewidth]{section-cellandrotorloading/FillingExtFillCellsSealingFillingHoles.png} \caption{Cell loading techniques (panel A) and the sealing of the filling holes of a cell (panel B).}\label{fig-cltsc}